Disciplina Discipline RBQ5781
Bases Moleculares da Regulação Gênica - Ênfase em Controle Pós-Transcricional

Molecular Basis of Gene Regulation – emphasis in post-transcriptional regulation

Área de Concentração: 17131

Concentration area: 17131

Criação: 03/12/2019

Creation: 03/12/2019

Ativação: 03/12/2019

Activation: 03/12/2019

Nr. de Créditos: 8

Credits: 8

Carga Horária:

Workload:

Teórica

(por semana)

Theory

(weekly)

Prática

(por semana)

Practice

(weekly)

Estudos

(por semana)

Study

(weekly)

Duração Duration Total Total
1 2 3 20 semanas 20 weeks 120 horas 120 hours

Docentes Responsáveis:

Professors:

Vanderlei Rodrigues

Tie Koide

Fausto Bruno dos Reis Almeida

María Eugenia Guazzaroni

Objetivos:

Apresentar aos estudantes de pós-graduação conceitos fundamentais e atualizações na área de regulação da expressão gênica, incluindo a utilização de novas tecnologias para o estudo de diversos mecanismos, desde a organização da cromatina a regulação pós-traducional. Noções de regulação pré-transcricional.

Objectives:

To present fundamental concepts and up-to-date information in gene regulation, including the use of new technologies to study diverse molecular mechanisms, from chromatin organization to post-translational control.

Justificativa:

Esta área do conhecimento tem avançado rapidamente e consequentemente, vem apresentando novos conceitos e mecanismos que dependem de um conhecimento básico aliado a atualizações e novas abordagens no estudo da regulação gênica. Para alcançar o objetivo de oferecer ao aluno uma noção da progressão e avanços centrais do conhecimento nessa área serão apresentados e discutidos artigos com algumas das descobertas seminais que representam “milestones” na compreensão de mecanismos e fatores centrais ao controle da expressão gênica. Em paralelo, um artigo que aborde algum avanço relevante na fronteira do conhecimento atual no mesmo tema será discutido.

Rationale:

The understanding of gene regulatorion has advanced rapidly in the last years, presenting new concepts and mechanisms that require a basic knowledge associated with up-to-date information and new technological approaches. This course aims to present a basic knowledge about the main advances in gene regulation, from seminal discoveries to more recent advances that are expanding the frontiers of gene regulation.

Conteúdo:

Visão geral da regulação transcricional Regulação pós-transcricional (modificações, splicing, RNAs regulatórios) Tradução e sua regulação Regulação pós-traducional Estocasticidade da expressão gênica

Content:

- Transcriptional regulation - review - Post-transcriptional regulation – RNA half-lives, regulatory RNAs - Translation - Post-translational control - Stochasticity in gene expression

Forma de Avaliação:

Prova escrita Apresentação e discussão de artigos científicos

Type of Assessment:

Studentes will be evaluated according to their involvement in classroom discussions and in area-sepecific seminars.

Bibliografia:

Genes - Benjamin Lewin , Oxford University Press Molecular Biology of the cell Molecular Biology of the Gene, Watson, sexta edição, CSHL Press Artigos dos avanços recentes extraídos de periódicos de alto impacto como: Cell, Nature, Science, Genes and Development, Nature Medicine, Nature genetics, Nature Cell Biology. Os artigos de fronteira serão escolhidos anualmente. Abaixo, alguns exemplos de artigos de revisões e artigos seminais a serem discutidos. Coupling and coordination in gene expression processes: a systems biology view - Komili S, Silver PA. Nat Rev Genet. 2008 Jan;9(1):38-48 Intron sequences involved in lariat formation during pre-mRNA splicing. Reed R, Maniatis T. (1985) Cell; 41(1): 95-105. Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches. Chen M, Manley JL. (2009). Nat Rev Mol Cell Biol.;10(11):741-54. All things must pass: contrasts and commonalities in eukaryotic and bacterial mRNA decay. Belasco JG (2010).Nat Rev Mol Cell Biol.;11(7):467-78 The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Nirenberg, M.W. and Matthaei, J.H. (1961) Proc. Natl. Acad. Sci. U. S. A. 47,1588–1602 General nature of the genetic code for proteins. Crick F H C, Barnett L, Brenner S, Watts-Tobin R J (1961) Nature 192: 1227-1232. Aminoacyl-tRNA synthesis and translational quality control. Ling J, Reynolds N, Ibba M.(2009) Annu Rev Microbiol. 63:61-78. Ribosome structure and dynamics during translocation and termination. Dunkle JA, Cate JH. (2010) Annu Rev Biophys. 9;39:227-44. Subcellular mRNA localization in animal cells and why it matters. Holt CE, Bullock SL. (2009) Science. 27;326(5957):1212-6. Functional roles for noise in genetic circuits. Eldar A, Elowitz MB. Nature. 2010 Sep 9;467(7312) Stochastic gene expression in a single cell. Elowitz MB, Levine AJ, Siggia ED, Swain PS. Science. 2002 Aug 16;297(5584):1183-6.

Bibliography:

Genes - Benjamin Lewin , Oxford University Press Molecular Biology of the cell Molecular Biology of the Gene, Watson, CSHL Press Articles about gene regulation from high-impact journals such as: Cell, Nature, Science, Genes and Development, Nature Medicine, Nature genetics, Nature Cell Biology. The articles will be selected specifically each year. Below, some examples of papers that were already used in previous editions of this course: Coupling and coordination in gene expression processes: a systems biology view - Komili S, Silver PA. Nat Rev Genet. 2008 Jan;9(1):38-48 Intron sequences involved in lariat formation during pre-mRNA splicing. Reed R, Maniatis T. (1985) Cell; 41(1): 95-105. Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches. Chen M, Manley JL. (2009). Nat Rev Mol Cell Biol.;10(11):741-54. All things must pass: contrasts and commonalities in eukaryotic and bacterial mRNA decay. Belasco JG (2010).Nat Rev Mol Cell Biol.;11(7):467-78 The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Nirenberg, M.W. and Matthaei, J.H. (1961) Proc. Natl. Acad. Sci. U. S. A. 47,1588–1602 General nature of the genetic code for proteins. Crick F H C, Barnett L, Brenner S, Watts-Tobin R J (1961) Nature 192: 1227-1232. Aminoacyl-tRNA synthesis and translational quality control. Ling J, Reynolds N, Ibba M.(2009) Annu Rev Microbiol. 63:61-78. Ribosome structure and dynamics during translocation and termination. Dunkle JA, Cate JH. (2010) Annu Rev Biophys. 9;39:227-44. Subcellular mRNA localization in animal cells and why it matters. Holt CE, Bullock SL. (2009) Science. 27;326(5957):1212-6. Functional roles for noise in genetic circuits. Eldar A, Elowitz MB. Nature. 2010 Sep 9;467(7312) Stochastic gene expression in a single cell. Elowitz MB, Levine AJ, Siggia ED, Swain PS. Science. 2002 Aug 16;297(5584):1183-6

Tipo de oferecimento da disciplina:

Presencial

Class type:

Presencial