Disciplina Discipline VCI5779
Células Tronco e Terapia Celular
Stem Cells and Cell Therapy

Área de Concentração: 10132

Concentration area: 10132

Criação: 21/12/2022

Creation: 21/12/2022

Ativação: 21/12/2022

Activation: 21/12/2022

Nr. de Créditos: 4

Credits: 4

Carga Horária:

Workload:

Docentes Responsáveis:

Professors:

Maria Angélica Miglino

Amanda Baracho Trindade Hill

Objetivos:

A disciplina Células Tronco e Terapia Celular tem por objetivo capacitar pós-graduandos e pesquisadores, na arte do conhecimento sobre células tronco de diferentes fontes teciduais e diferentes potencialidades, clonagem animal terapêutica, uso clínico das células tronco nas diferentes patologias que acometem os animais, de modo a discutir propriedades, funções, mecanismos de ação e eficácia das células tronco e terapia celular, além de interagir e integrar tópicos sobre regulamentação e ética.

Objectives:

The Stem Cells and Cell Therapy course will qualify post-graduate students and researchers in the art of deriving and preparing stem cells from diverse tissue sources for the fulfillment of therapeutic and research objectives in animal cloning and veterinary pathology through the discussion of the properties, functions, mechanisms of action, and efficacy of stem cells and cell therapy, as well as provide an integrated perspective on ethics and current regulations.

Justificativa:

Os avanços na genética e biologia molecular vêm provocando mudanças significativas nas áreas da Medicina Veterinária. Os últimos dez anos marcaram a expansão do conhecimento sobre as células-tronco e suas aplicações clínicas. Descobertas no campo da medicina regenerativa revelam a potencialidade dessas células e a gama de aspectos que podem ser explorados por jovens pesquisadores, para isso os mesmos necessitam de conhecimento prévio da potencialidade, da obtenção nos diferentes tecidos e aplicabilidade das mesmas. Apesar das limitações impostas pela moral, pela ética e pelo senso comum, assim como a aplicação das células tronco, a clonagem é um campo importante, cujos benefícios para a humanidade são muitos. Deste modo, a disciplina oferecida prevê instruir os alunos com o conhecimento necessário para sua aplicabilidade futura.

Rationale:

Advances in genetics and molecular biology have potentiated significant changes in the practice of veterinary medicine. The last ten years have been witness to a landmark expansion in knowledge regarding stem cells and their clinical applications. Discoveries in the field of regenerative medicine have revealed the potential that stem cells have and their wide range of attributes waiting to be explored by young researchers. These researchers are prevented from engaging in this emerging field due to their lack of experience and prior knowledge, specifically in regard to the derivation of different types of stem cells and the applications for which each type is best suited. Despite ethical, moral, and practical limitations, cloning, like stem cell application, is an important field from which humanity stands to benefit greatly. As such, this course intends to provide students with the necessary knowledge base for its future implementations.

Conteúdo:

1. Células tronco: aspectos gerais e classificação; 2. Desenvolvimento embrionário inicial; 3. Clonagem Animal; 4. Células tronco pluripotente induzidas; 5. Fontes de obtenção de células tronco no organismo; 6. Células tronco adultas; 7. Bases da terapia celular; 8. Terapia celular aplicada às diferentes patologias que acometem os animais; 9. Bioética e regulamentação no uso de células tronco e clonagem animal.

Content:

1. Stem cells: background and classification 2. Early embryo development; 3. Animal cloning; 4. Induced pluripotent stem cells; 5. Sources of stem cells; 6. Adult stem cells; 7. Basis of stem cell therapy; 8. Cell therapy applied to veterinary pathologies; 9. Bioethics and regulations regarding stem cells use and animal cloning.

Forma de Avaliação:

A avaliação será realizada mediante apresentação de seminário, cuja nota (0-10) determinará o conceito final (A,B,C,R), considerando os respectivos intervalos: 10-9 (A); 8-7 (B); 6-5 (C); 4-0 (R).

Type of Assessment:

The evaluation will be carried out by presentation of a seminar, whose score (0-10) will determine the final concept (A,B,C,R), considering the respective intervals: 10-9 (A); 8-7 (B); 6-5 (C); 4-0 (R).

Bibliografia:

Batchelder CA, Hoffert KA, Bertolini M, Moyer AL, Mason JB, Petkov SG, Famula TR, Anderson GB. Effect of the nuclear-donor cell lineage, type, and cell donor on development of somatic cell nuclear transfer embryos in cattle. Cloning Stem Cells, 2005, no prelo. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dal Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KHS. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 2000; 407:86-89. ATALA, Anthony et al. (Ed.). Principles of regenerative medicine. Academic press, 2018. MUKHOPADHYAY, Asok (Ed.). Regenerative Medicine: Laboratory to Clinic. Springer Singapore, 2017. GUGJOO, Mudasir B. et al. Mesenchymal stem cell research in veterinary medicine. Current stem cell research & therapy, v. 13, n. 8, p. 645-657, 2018. JAYARAMAN, Premkumar et al. Acceleration of translational mesenchymal stromal cell therapy through consistent quality GMP manufacturing. Frontiers in cell and developmental biology, v. 9, p. 648472, 2021. GUGJOO, Mudasir B. et al. Mesenchymal stem cell research in veterinary medicine. Current stem cell research & therapy, v. 13, n. 8, p. 645-657, 2018. BOGERS, Sophie H.; BARRETT, Jennifer G. Veterinary Medicine’s Advances in Regenerative Orthopedics. In: Metabolic Therapies in Orthopedics, Second Edition. CRC Press, 2018. p. 59-91. VIKARTOVSKA, Zuzana et al. Adult Stem Cells Based Therapies in Veterinary Medicine. Archives of Veterinary Science and Medicine, v. 3, n. 2, p. 40-50, 2020. VILLATORO JIMÉNEZ, A. J. et al. Regenerative medicine in ocular surface pathologies in veterinary ophthalmology. Argos-Informativo Veterinario, n. 196, p. 70-74, 2018. DIAS, Inês Esteves et al. Mesenchymal stem cells therapy in companion animals: useful for immune-mediated diseases?. BMC veterinary research, v. 15, n. 1, p. 1-14, 2019. DAMAYANTI, Restu Harisma; RUSDIANA, Taofik; WATHONI, Nasrul. Mesenchymal stem cell secretome for dermatology application: a review. Clinical, Cosmetic and Investigational Dermatology, v. 14, p. 1401, 2021. KUMAR, Vinod; HANDA, Sanjeev. Stem Cells in Dermatology: What the Future May Hold. In: Biomedical Translational Research. Springer, Singapore, 2022. p. 13-34. KRAIŃSKA, Magdalena M. et al. Extracellular vesicles derived from mesenchymal stem cells as a potential therapeutic agent in acute kidney injury (AKI) in felines: review and perspectives. Stem cell research & therapy, v. 12, n. 1, p. 1-16, 2021. DIOMAIUTO, Emanuela et al. Exosomes in Dogs and Cats: An Innovative Approach to Neoplastic and Non-neoplastic Diseases. Pharmaceuticals, v. 14, n. 8, p. 766, 2021. MARCHEQUE, Julia et al. Concise reviews: stem cells and kidney regeneration: an update. Stem cells translational medicine, v. 8, n. 1, p. 82-92, 2019. GUGJOO, Mudasir Bashir. Mesenchymal Stem Cells Therapeutic Applications in Gastrointestinal Disorders. In: Therapeutic Applications of Mesenchymal Stem Cells in Veterinary Medicine. Springer, Singapore, 2022. p. 247-278. HASSAN, Safaa A. et al. Therapeutic efficiency of adipose-derived mesenchymal stem cells in healing of experimentally induced gastric ulcers in rats. Anatomy & Cell Biology, v. 54, n. 3, p. 361-374, 2021. CAIN, Christine L. Canine perianal fistulas: clinical presentation, pathogenesis, and management. Veterinary Clinics: Small Animal Practice, v. 49, n. 1, p. 53-65, 2019. ADAMIČ, Neža et al. Effect of intrabronchial administration of autologous adipose-derived mesenchymal stem cells on severe equine asthma. Stem cell research & therapy, v. 13, n. 1, p. 1-14, 2022. ZHANG, Li-Bo; HE, Min. Effect of mesenchymal stromal (stem) cell (MSC) transplantation in asthmatic animal models: a systematic review and meta-analysis. Pulmonary pharmacology & therapeutics, v. 54, p. 39-52, 2019. GAO, Lei et al. Cardio-renal exosomes in myocardial infarction serum regulate proangiogenic paracrine signaling in adipose mesenchymal stem cells. Theranostics, v. 10, n. 3, p. 1060, 2020. CATS, PURE RED CELL APLASIA IN. PURE RED CELL APLASIA IN DOGS Pathogenesis Pure red cell aplasia is an uncommon disorder and causes severe chronic nonregenerative anemia and clini-cal signs similar to PIMA. Most reported cases of PRCA. Schalm's Veterinary Hematology, p. 309, 2022. COMIZZOLI, Pierre; HOLT, William V. Breakthroughs and new horizons in reproductive biology of rare and endangered animal species. Biology of Reproduction, v. 101, n. 3, p. 514-525, 2019. GHAHREMANI‐NASAB, Maryam et al. Premature ovarian failure and tissue engineering. Journal of cellular physiology, v. 235, n. 5, p. 4217-4226, 2020. LANGE-CONSIGLIO, Anna et al. Case report: Use of amniotic microvesicles for regenerative medicine treatment of a mare with chronic endometritis. Frontiers in Veterinary Science, v. 7, p. 347, 2020. CAPLAN, Arnold I. Mesenchymal stem cells: time to change the name!. Stem cells translational medicine, v. 6, n. 6, p. 1445-1451, 2017.

Bibliography:

Batchelder CA, Hoffert KA, Bertolini M, Moyer AL, Mason JB, Petkov SG, Famula TR, Anderson GB. Effect of the nuclear-donor cell lineage, type, and cell donor on development of somatic cell nuclear transfer embryos in cattle. Cloning Stem Cells, 2005, no prelo. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dal Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KHS. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 2000; 407:86-89. ATALA, Anthony et al. (Ed.). Principles of regenerative medicine. Academic press, 2018. MUKHOPADHYAY, Asok (Ed.). Regenerative Medicine: Laboratory to Clinic. Springer Singapore, 2017. GUGJOO, Mudasir B. et al. Mesenchymal stem cell research in veterinary medicine. Current stem cell research & therapy, v. 13, n. 8, p. 645-657, 2018. JAYARAMAN, Premkumar et al. Acceleration of translational mesenchymal stromal cell therapy through consistent quality GMP manufacturing. Frontiers in cell and developmental biology, v. 9, p. 648472, 2021. GUGJOO, Mudasir B. et al. Mesenchymal stem cell research in veterinary medicine. Current stem cell research & therapy, v. 13, n. 8, p. 645-657, 2018. BOGERS, Sophie H.; BARRETT, Jennifer G. Veterinary Medicine’s Advances in Regenerative Orthopedics. In: Metabolic Therapies in Orthopedics, Second Edition. CRC Press, 2018. p. 59-91. VIKARTOVSKA, Zuzana et al. Adult Stem Cells Based Therapies in Veterinary Medicine. Archives of Veterinary Science and Medicine, v. 3, n. 2, p. 40-50, 2020. VILLATORO JIMÉNEZ, A. J. et al. Regenerative medicine in ocular surface pathologies in veterinary ophthalmology. Argos-Informativo Veterinario, n. 196, p. 70-74, 2018. DIAS, Inês Esteves et al. Mesenchymal stem cells therapy in companion animals: useful for immune-mediated diseases?. BMC veterinary research, v. 15, n. 1, p. 1-14, 2019. DAMAYANTI, Restu Harisma; RUSDIANA, Taofik; WATHONI, Nasrul. Mesenchymal stem cell secretome for dermatology application: a review. Clinical, Cosmetic and Investigational Dermatology, v. 14, p. 1401, 2021. KUMAR, Vinod; HANDA, Sanjeev. Stem Cells in Dermatology: What the Future May Hold. In: Biomedical Translational Research. Springer, Singapore, 2022. p. 13-34. KRAIŃSKA, Magdalena M. et al. Extracellular vesicles derived from mesenchymal stem cells as a potential therapeutic agent in acute kidney injury (AKI) in felines: review and perspectives. Stem cell research & therapy, v. 12, n. 1, p. 1-16, 2021. DIOMAIUTO, Emanuela et al. Exosomes in Dogs and Cats: An Innovative Approach to Neoplastic and Non-neoplastic Diseases. Pharmaceuticals, v. 14, n. 8, p. 766, 2021. MARCHEQUE, Julia et al. Concise reviews: stem cells and kidney regeneration: an update. Stem cells translational medicine, v. 8, n. 1, p. 82-92, 2019. GUGJOO, Mudasir Bashir. Mesenchymal Stem Cells Therapeutic Applications in Gastrointestinal Disorders. In: Therapeutic Applications of Mesenchymal Stem Cells in Veterinary Medicine. Springer, Singapore, 2022. p. 247-278. HASSAN, Safaa A. et al. Therapeutic efficiency of adipose-derived mesenchymal stem cells in healing of experimentally induced gastric ulcers in rats. Anatomy & Cell Biology, v. 54, n. 3, p. 361-374, 2021. CAIN, Christine L. Canine perianal fistulas: clinical presentation, pathogenesis, and management. Veterinary Clinics: Small Animal Practice, v. 49, n. 1, p. 53-65, 2019. ADAMIČ, Neža et al. Effect of intrabronchial administration of autologous adipose-derived mesenchymal stem cells on severe equine asthma. Stem cell research & therapy, v. 13, n. 1, p. 1-14, 2022. ZHANG, Li-Bo; HE, Min. Effect of mesenchymal stromal (stem) cell (MSC) transplantation in asthmatic animal models: a systematic review and meta-analysis. Pulmonary pharmacology & therapeutics, v. 54, p. 39-52, 2019. GAO, Lei et al. Cardio-renal exosomes in myocardial infarction serum regulate proangiogenic paracrine signaling in adipose mesenchymal stem cells. Theranostics, v. 10, n. 3, p. 1060, 2020. CATS, PURE RED CELL APLASIA IN. PURE RED CELL APLASIA IN DOGS Pathogenesis Pure red cell aplasia is an uncommon disorder and causes severe chronic nonregenerative anemia and clini-cal signs similar to PIMA. Most reported cases of PRCA. Schalm's Veterinary Hematology, p. 309, 2022. COMIZZOLI, Pierre; HOLT, William V. Breakthroughs and new horizons in reproductive biology of rare and endangered animal species. Biology of Reproduction, v. 101, n. 3, p. 514-525, 2019. GHAHREMANI‐NASAB, Maryam et al. Premature ovarian failure and tissue engineering. Journal of cellular physiology, v. 235, n. 5, p. 4217-4226, 2020. LANGE-CONSIGLIO, Anna et al. Case report: Use of amniotic microvesicles for regenerative medicine treatment of a mare with chronic endometritis. Frontiers in Veterinary Science, v. 7, p. 347, 2020. CAPLAN, Arnold I. Mesenchymal stem cells: time to change the name!. Stem cells translational medicine, v. 6, n. 6, p. 1445-1451, 2017.

Tipo de oferecimento da disciplina:

Presencial

Class type:

Presencial